Screening of Antioxidant, Antimicrobial and Phytochemicals Composition of Various Plant Parts of Cichorium Intybus

Pharmaceutical Science-Botany for better natural therapy

Abstract

The present study was conducted to evaluate the antioxidant and antimicrobial potential as well as the phytochemical composition of root, seed and leaves of Cichoriumintybus. The antioxidant activity was analyzed by detecting the 2, 2-Diphenyl-1- picrylhydrazyl  DPPH  radical  scavenging  potent  of  the constituents found  in  various  plant  parts  of Cichorium intybus,whereas for analyzing in-vitro antimicrobial  activity, agar well diffusion method has been opted. The antimicrobial  activity of plant was tested against both pathogenic as well as non-pathogenic species of bacteria by analyzing the corresponding inhibition zone exhibited by various  extracts  of  plant  parts  against  the  bacterial  growth.  Apart  from  this,  the  present  study  also  involves  the  quantitative phytochemical evaluation of total ascorbic acid in root, seed and leaves of Cichoriumintybus. The antioxidant potential of the plant was found to be comparatively lower in seed and leaves of plant than in root in accordance to the determined IC50 value . From the antimicrobial activity analysis, it was concluded that majority of times the non-polar extracts of all plant parts were found to be more effective  in inhibiting  growth  of bacteria.  Most significant  activity  has been  exhibited against  gram negative  bacteria  Pseudomonas aeruginosa. The ascorbic acid content in plant was found in the range of    2.253±0.867 to 12.594±2.05µg/ml. From the results of the conducted research, the importance  of plant as an antimicrobial and anti-oxidative  agent has been established. So, the plant can further be introduced as a potential drug for herbal medicine in the treatment of human disorders.