Characterization of Purified Collagen from Marine Squid Uroteuthis Duvauceli

Life Sciences-Biotechnology for Prospective Medical Science

Abstract

Collagen is the essential and most abundant structural protein found in the animal kingdom, involving almost 25-30% of the entire body protein  content.  It is normally  found in fibrous tissues,  for example,  skin, tendons,  ligaments,  skin, corneas, ligament, bones, veins, teeth, and so on. The skin of the squid is rich in collagen and is an excellent source of raw materials  that  can  be used  for the  development  of  collagen  products.  As such, the  use  of Uroteuthis  duvauceli  skin is  an alternative source of collagen may be an effective way to obtain high-value-added products. In this study, - the isolated Type 1 collagen was found in Uroteuthis duvauceli (skin and muscle ). Additionally, the characterization of the Type 1 collagen from Uroteuthis duvauceli (skin and muscle ) was examined through various techniques  such as SEM, FTIR, XRD, TGA. The skin and muscle showed the typical SDS-PAGE pattern of type I collagen with two different α bands, α1 and α2 and also contains β and γ chains. The fibrillar structures of collagen samples were observed by SEM. FTIR investigation showed the existence of helical arrangements of the type 1 collagen from skin and muscle. UV spectrum of Type1 collagen from Uroteuthis duvauceli skin and muscle has a maximum absorption peak near 230 nm and a minor absorption peak near 280 nm. In XRD, type1 collagen from Uroteuthis duvauceli skin and muscle one wide peak was obtained following with characteristic diffraction peaks of collagen. The results showed that type 1 collagen from skin and muscle had slight differences in molecular weight, amino acid composition, morphological structures, and thermal stability. The present data on collagens from the marine squid, could help the future endeavors to unwind the therapeutically significant, more secure collagens from marine squid for their utilization in biomedical field.