VANCOMYCIN RESISTANT STAPHYLOCOCCUS AUREUS INDUCED OXIDATIVE STRESS IN LIVER AND KIDNEY: PROTECTIVE ROLE OF NANOCONJUGATED VANCOMYCIN

Pharmaceutical Science-Chemistry

Abstract

Staphylococcus aureus is a most common pathogen in hospital and community acquired disease that causes a wide range of infection such as skin and soft tissue infection to life threatening disease like respiratory tract infection, musculoskeletal infection, endocarditis and urinary tract infection. The aim of the present study was to evaluate the possible protective effects of nanoconjugated vancomycin against VRSA infection on selective makers of oxidative damage and antioxidant status in liver and kidney. A coagulase positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 X 106 CFU/ml bacterial solutions. Nanoconjugated vancomycin was treated to VRSA infected mice at its effective dose for 10 days. After decapitation, liver and kidney were excised from control and experimental groups, homogenized and used for different biochemical estimation. Nitrate level, myeloperoxidse activity, lipid peroxidation, protein oxidation, oxidized glutathione, DNA fragmentation level were increased significantly (p<0.05) in liver and kidney of VRSA infected group as compared to control group, and reduced glutathione level, activity of antioxidant enzymes (SOD and CAT), glutathione dependent enzymes (GPx, GR and GST) were decreased significantly (p<0.05); which were increased or decreased significantly (p<0.05) near to normal in nanoconjugated vancomycin treated group. These finding suggests the potential use and beneficial role of nanoconjugated vancomycin against VRSA infection induced oxidative stress and DNA damage in liver and kidney.