Antagonists Targeting miR-155 and miR-125a Relieved Ultrasonographic Contrast Medium SonoVue Induced Kidney Injury
Pharmaceutical sciences- Pharma
DOI:
https://doi.org/10.22376/ijlpr.2023.13.6.P310-P317Keywords:
kidney injury, ultrasonographic contrast medium, miR-155 and miR-125a antagonists, NF-kappa B signalingAbstract
Ultrasonographic contrast medium (UCM) enhances radiological procedures. However, it raises a serious problem thatUCM induces acute kidney injury. However, the mechanism of kidney injury induced by ultrasonographic contrast medium remainselusive. This work aims to explore kidney injury from a post-transcription view. UCM was identified to induce kidney injury byactivating NF-kappa B signaling. The expression of NF-kappa B was examined in mouse kidneys by western blot. The level of NFkappa B was upregulated in the kidney with the treatment of UCM. Hereof, the expression levels of miR-155 and miR-125a,upstream of NF-kappa B, increased significantly in the UCM group, which RT-PCR detects. As a positive control, LPS was used toinduce acute kidney injury. The expressions of NF-kappa B, miR-155, and miR-125a all increased in the LPS group. Further, to verifythe necessity of NF-kappa B signaling in the process of UCM-induced kidney injury, the mice were treated with the antagonists ofmiR-155 and miR-125a, and our results showed that the antagonists of miR-155 and miR-125a repressed NF-kappa B signaling bywestern blot analysis. In conclusion, our results demonstrate that miR-155 and miR-125a antagonists mediate NF-kappa B signalingto relieve kidney injury, which UCM induced. The antagonists would be further tested to alleviate kidney injury clinically.
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