Development and Characterization of Griseofulvin Nanosponges to Enhance Bioavailability
Pharmaceutical Science-Pharmaceutics
DOI:
https://doi.org/10.22376/ijpbs/lpr.2022.12.5.P99-111Keywords:
Griseofulvin, Cyclodextrin, Nanosponges, Oral Bioavailability, Emulsion Solvent Dispersion MethodAbstract
The pharmaceutical industries are more interested in development of novel drug delivery system to overcome the disadvantages of traditional drug delivery system. Now a days, fungal infections are increasing worldwide. Hence the demand of novel dosage form is increased with improved therapeutic effect and minimum side effect. The main aim of this study was to develop griseofulvin loaded ß -cyclodextrin based nanosponges for improving dissolution, oral bioavailability. In the present study, the griseofulvin loaded Nano sponges were prepared by emulsion solvent diffusion method. The six different formulation batches were formulated with varying concentration ratio of drug and polymer. The Preformulation study of the drug was done by UVvisible spectrophotometer. Drug, polymer and excipient compatibility studies were analyzed by FTIR studies. The prepared Nanosponges formulation was evaluated for particle size, zeta potential, polydispersity index, scanning electron microscopy and in-vitro drug release studies. The SEM studies showed the highly porous structure of griseofulvin loaded nanosponges having a sponges-like shape. The entrapment efficiency of optimized batch F4 was found to be 97.69±0.006%. The particle size and zeta potential of optimized batch F4 was found to be 488.59nm and -20.77 respectively. The in-vitro drug release of optimized batch F4 showed the maximum drug release of 75.56% in 8 hrs. The current study successfully developed griseofulvin loaded ß - cyclodextrin based nanosponges to improve dissolution rate, oral bioavailability and masking bitter taste. The polymer used in preparation of nanosponges shows efficient drug release. Griseofulvin loaded nanosponges drug delivery showed prolonged release which is beneficial for chronic fungal infection. Another advantage of formulating novel dosage forms is reduction in dose, dosing frequency and reduced side effects.
References
Dash AK, Mishra D. Method development, validation and stability study of griseofulvin in bulk and pharmaceutical dosage form by spectrophotometric method. Asian J Pharm Res. 2012;2(1):66-9.
Bennett ML, Fleischer Jr AB, Loveless JW, Feldman SR. Oral griseofulvin remains the treatment of choice for tinea capitis in children. Pediatr Dermatol. 2000;17(4):304-9. doi: 10.1046/j.1525-1470.2000.01784.x, PMID 10990583.
Amidon GL, Lennernäs H, Shah VP, Crison JR. A theoretical basis for a biopharmaceutic drug classification: the correlation of in vitro drug product dissolution and in vivo bioavailability. Pharm Res. 1995;12(3):413-20. doi: 10.1023/a:1016212804288, PMID 7617530.
Reverchon E, Della Porta GD, Spada A, Antonacci A. Griseofulvin micronization and dissolution rate improvement by supercritical assisted atomization. J Pharm Pharmacol. 2004;56(11):1379-87. doi: 10.1211/0022357044751, PMID 15525444.
Pattnaik S, Swain K, Venkateshwar Rao J. Nanosuspension: A strategy for improved bioavailability. Int J Pharm Biol Sci. 2013;3(4):324-7.
Arida AI, Al-Tabakha MM, Hamoury HAJ. Improving the high variable bioavailability of griseofulvin by SEDDS. Chem Pharm Bull (Tokyo). 2007;55(12):1713-9. doi: 10.1248/cpb.55.1713, PMID 18057745.
S S, S A, Krishnamoorthy K, Rajappan MNanosponges: a novel class of drug delivery system–review. J Pharm Pharm Sci. 2012;15(1):103-11. doi: 10.18433/j3k308. PMID 22365092.
Ansari KA, Vavia PR, Trotta F, Cavalli R. Cyclodextrin-Based Nanosponges for Delivery of Resveratrol: In Vitro Characterisation, Stability, Cytotoxicity and Permeation Study. AAPS PharmSciTech. 2011;12(1):279-86. doi: 10.1208/s12249-011-9584-3.
Ahuja A, Baboota S, Ali J, Mustafa G. Cyclodextrins as potential excipients in pharmaceutical formulations: solubilizing and stabilizing effects. Cyclodextrins Pharm Cosmet Biomed Curr Fut Ind Appl. 2011:19-43.
Cavalli R, Trotta F, Tumiatti W. Cyclodextrin-based nanosponges for drug Delivery. J Incl Phenom Macrocycl Chem. 2006;56(1-2):209-13. doi: 10.1007/s10847-006-9085-2.
Rohini B, Shivappa N, Nasheer S, Avinash S. Formulation and evaluation of nanosponges hydrogel for Topical drug delivery containing griseofulvin. Int J Med Pharm Sci. 2020;10:57-70.
Shelake SS, Patil SV, Patil SS. Formulation and Evaluation of fenofibrate- loaded Nanoparticles by Precipitation Method. pharmaceutical-sciences. 2018;80(3):420-7. doi: 10.4172/pharmaceutical-sciences.1000374.
Ghurghure SM, Ka K. Thorat Ys, Pathan ma. Preparation and in-vitro evaluation of itraconazole loaded nanosponges for topical drug delivery.Indo-. Am J Pharm Res, 2019.1999-2013.
The Indian pharmacopeia, government of India, ministry of health and family welfare, published by the Indian pharmacopeia commission, Ghaziabad. 2010;2:1540-1541:A117-24.
Priyanka D, Sindhu S. Design and development of ibuprofen loaded nanosponges. Int J ChemTech Res. 2018;11.
Rao MRP, Bhingole RC. Nanosponge-based pediatric-controlled release dry suspension of Gabapentin for reconstitution. Drug Dev Ind Pharm. 2015;41(12):2029-36. doi: 10.3109/03639045.2015.1044903.
Furniss BS. Vogel’s textbook of practical organic chemistry. Pearson; 1989.
Dr. Pratima Sriniwas SK, et al. Formulation and evaluation of voriconazole loaded nanosponges for oral and topical delivery. Int J Drug Dev Res. 2013;5(1):55-69.
Pawar AY, Aurangabadkar VM, et al. Formulation development and evaluation of topical microemulsion gels for nimsulide. J Pharm Res. 2011;4(4):1004-6.
Pandey J, singh a et al. Formulation and evaluation of nanosponges based controlled release gel preparation of ketoconazole. Int J Pharm Pharm Res. 2018;12(3):367-82.
Shaikh AN, Pawar AY. Formulation and evaluation nanosponges loaded hydrogel of luliconazole. IJSDR ISSN. 2020;5(8, August):2455-631.
Aggarwal G, Nagpal M, Kaur G. Development and Comparison of Nanosponge and Niosome based Gel for the Topical Delivery of Tazarotene. Pharm Nanotechnol;4(3):213-28. doi: 10.2174/2211738504666160804154213.
Thimmaraju MK, Venkat Rao SG. G Jayappal reddy.UV Spectrometric method for determination of finasteride in bulk and pharmaceutical dosage form. Int J Pharm Biol Sci. 2011;1(1):39-43.
Pavia DL, Lampman GM. Spectroscopy. Infrared Spectroscopy. Cengage Learning. 2007;26-107.
Omar SM, Ibrahim F, Ismail A. Formulation and evaluation of cyclodextrin-based nanosponges of griseofulvin as pediatric oral liquid dosage form for enhancing bioavailability and masking bitter taste. Saudi Pharm J. 2020;28(3):349-61. doi: 10.1016/j.jsps.2020.01.016, PMID 32194337.
Srinivas P, Sreeja K. Formulation and evaluation of voriconazole loaded nanosponges for oral and topical delivery. Int J Drug Deliv Res. 2013;5(1):55-69.
Manyam N, Kumar K. Formulation and Evaluation of Nanosponges Loaded extended release of trimethoprim, J Pharma Med Hea sci. 2018;1(1):78-86.
Kumar S, Hematheerthan N, Ratan J. Design and characterization of miconazole nitrate loaded nanosponges containing vaginal gel. Int J Pharm Ana Res. 2016;5(3):410-7.
Kehserwani R, Sachan A, Arora M. Formulation and Evaluation of Solid-Lipid Nanoparticle (SLN) Based Topical gel of Etoricoxib. J Appl Pharm Sci. 2016;4(1):124-31.
Shringirishi M, Mahor A, Gupta R, Prajapati SK, Bansal K, Kesharwani P. Fabrication and characterization of nifedipine loaded β-cyclodextrin nanosponges: an in vitro and in vivo evaluation. J Drug Deliv Sci Technol. 2017;41:344-50. doi: 10.1016/j.jddst.2017.08.005.
Published
How to Cite
Issue
Section
Copyright (c) 2022 Ashish Y. Pawar, Khanderao R. Jadhav, Jyoti B. Rao, Ashvini D. Tapkir, Prashant S. Malpure, Rishikesh S. Bachhav
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
