International Journal of Life science and Pharma Reviews (IJLPR)  
   
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Article
Pharmaceutical Sciences
Volume 12 Issue 3, May 2022    Pages:86-96
Histomorphological Progression of Diethylnitrosamine (DEN) Induced Hepatocellular Carcinoma Model in Rat  

Kunal Mukherjee, Souvik Biswas, Dipan Roy, Payel Laha, Nabanita Pramanick and Arijit Das
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DOI: 10.22376/ijpbs/lpr.2022.12.3.P86-96
Abstract:
Hepatocellular carcinoma (HCC) commonly known as hepatoma is a frequently occurring liver cancer that occupies 75% of all types of liver cancers. It affects the hepatocellular cells which is the major cell of the liver. Some models can be developed for HCC in rats but it is time-consuming. Nitrosodiethylamine is a very popular carcinogenic drug, very useful to develop experimental animal models. The present study focuses on the establishment of an animal model through a simple approach of experimental protocol along with the duration of the experiment that leads to creating a scope for future studies associated with metastasis as well as anti-metastasis studies. For the present study 2 groups were formed where each group containing 16 rats. Phenobarbital was given for 7 days to each group after diluting with water at a concentration of 0.3g/L before administering the first DEN. The control group received the same number of vehicles for 8 weeks. 50mg/kg of DEN was received intraperitoneally by the animal of the 2nd group twice a week for eight weeks. Bodyweight, the weight of the liver, and all the blood parameters were analysed like alpha-fetoprotein, SGPT, SGOT, and ALP, and a comparison was made with the control group. After evaluating all biological and histological data it can be concluded that the outcome of the present study signifies a simple experimental protocol along with an appropriate experimental duration animal model which leads to help in the study of the mechanism of metastasis and anti-metastasis agent in near future. The current work also represents a straightforward experimental methodology that aids in the investigation of metastatic mechanisms and anti-metastasis agents, with phenobarbital serving as a good promoting agent.
Keywords: Hepatocellular Carcinoma (HCC), DMN, DEN, Phenobarbital, SGPT, SGOT, ALP, Anti-Metastasis Agents
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