International Journal of Life science and Pharma Reviews (IJLPR)  
International Journal of Life science and Pharma Research (IJLPR)
Pharmaceutical Sciences
Volume 10 issue 2, April - June 2020    Pages:26-33
Quantification of Atazanavir and Ritonavir in Human Plasma Samples by Rp-Hplc Include Method of Detection in the Title, Eg: Using PDA Detection

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A fixed oral dose combination of Atazanavir and Ritonavir is currently used for the treatment of patients with HIV infections. A selective and novel bio-analytical technique was designed to evaluate Atazanavir and Ritonavir by mass spectroscopic investigation in plasma matrices. The method was chromatographed with Agilent TC-C18, 4.6 x 75 mm, 3.5 μm, 80 Å column, 5mM ammonium acetate: acetonitrile (20:80 v/v) mobile phase was used for Chromatographic separation. UV detector was used to detect the Atazanavir and Ritonavir at 235 nm. For extraction of the analyte and internal standard, Liquid-liquid extraction was employed .This method is validated over a linear concentration range of 50.0 – 10000.0 ng/ml for Atazanavir and Ritonavir with a correlation coefficient (r) of ≥ 0.9997 and both drugs were stable in plasma samples.
Keywords: HPLC; Atazanavir, Ritonavir, Human plasma; Bio analytical
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