International Journal of Life science and Pharma Reviews (IJLPR)  
   
 
International Journal of Life science and Pharma Research (IJLPR)
Life Science
Volume 6 Issue 4, October - December 2016    Pages:45-52
ISOLATION AND CHARACTERIZATION OF ANTIBODIES CHALLENGING STAPHYLOCOCCUS AUREUS USING JAPANESE QUAIL

IDA SHARON E, MR. LEVIN ANBU GOMEZ, C.VANI, A.MICHAEL
[View PDF]
DOI: DOI not available
Abstract:
This study focus on isolation and characterization of antibody (IgYs) which were isolated from the Japanese quail (Coturnix japonica) egg yolk, and its effect against S.aureus. Formalin inactivated S.aureus antigen were intramuscularly immunized in quail via thigh muscles. Then after 7th and 14th day of immunization the immunized eggs were collected. Eggs are used based on the conventional bleeding procedure of mammals. Egg yolk was separated and IgY antibodies were isolated, purified. Enzyme-Linked Immunosorbent Assay (ELISA) was used to analyze the activity of the highest antibody. The molecular weight of the IgY antibody is found to be 93KDa using SDS PAGE. The specificity was determined by using Immunoelectrophoresis. The presence of antibody against the particular antigen is observed by the Ouchterlony Double Diffusion method. The concentration of the antigen is determined by Single Radial Immuno Diffusion. The study validates the presence of antibodies in Japanese Quail thereby representing quail to be an alternative source of avian antibodies.
Keywords: Japanesequail, IgY, S.aureus, ELISA, SRID, ODD.
 
 
stripe
© 2010-2015 IJLPR. All rights reserved. Specialized online journals by ubijournal. Website by Ubitech Solutions